Journal article

Reversing diet-induced metabolic dysregulation by diet switching leads to altered hepatic de novo lipogenesis and glycerolipid synthesis

Greg M Kowalski, Steven Hamley, Ahrathy Selathurai, Joachim Kloehn, David P De Souza, Sean O'Callaghan, Brunda Nijagal, Dedreia L Tull, Malcolm J McConville, Clinton R Bruce



In humans, low-energy diets rapidly reduce hepatic fat and improve/normalise glycemic control. Due to difficulties in obtaining human liver, little is known about changes to the lipid species and pathway fluxes that occur under these conditions. Using a combination of stable isotope, and targeted metabolomic approaches we investigated the acute (7-9 days) hepatic effects of switching high-fat high-sucrose diet (HFD) fed obese mice back to a chow diet. Upon the switch, energy intake was reduced, resulting in reductions of fat mass and hepatic triacyl- and diacylglycerol. However, these parameters were still elevated compared to chow fed mice, thus representing an intermediate phenotype. Nonet..

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Awarded by National Health and Medical Research Council (NHMRC) of Australia

Awarded by NHMRC

Funding Acknowledgements

This work was supported by a National Health and Medical Research Council (NHMRC) of Australia grant to C.R.B (1023570) and a grant from the Diabetes Australia Research Trust to G.M.K. M.J.M. is supported by a fellowship from the NHMRC (APP1059530). G.M.K. and C.R.B. are the guarantors of this work and, as such, have full access to all the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis.