FACTORS AFFECTING PROTOPLAST ISOLATION AND THE REGENERATION OF SHOOT-LIKE STRUCTURES FROM PROTOPLAST-DERIVED CALLUS OF SUGARCANE (SACCHARUM SPP HYBRIDS)

Abstract

High yields of protoplasts were isolated from non-regenerable, homogeneous cell suspension cultures of sugarcane, compared with regenerable, heterogeneous cell suspension cultures after incubation in an enzyme composition containing Cellulase RS, Pectinase, Macerozyme and Driselase. Higher yields of protoplasts were released from heterogeneous cell suspension cultures after the addition of 10 mg L-1 silver nitrate to the culture medium; however, ethylene production was not involved in protoplast isolation. Use of 0-05-2% Pectolyase Y23 pectinase rather than other pectinases resulted in higher yields of protoplasts from heterogeneous cell suspension cultures. These results suggest that there ..