RESISTANCE OF MELANOMA CELL-LINES TO INTERFERONS CORRELATES WITH REDUCTION OF IFN-INDUCED TYROSINE PHOSPHORYLATION - INDUCTION OF THE ANTIVIRAL STATE BY IFN IS PREVENTED BY TYROSINE KINASE INHIBITORS

Abstract

Clinical and experimental studies examining the action of IFNs on human malignant melanomas and melanoma cell lines have shown that this cancer cell type is frequently IFN resistant. In the present study, the IFN responsiveness of five melanoma cell lines, SK-MEL-28, SK-MEL-3, MM96, HT-144, and Hs 294T, as determined by the levels of IFN-induced expression of the antiviral proteins, 100 kDa 2',5'-oligoadenylate synthetase (OAS) and Mx Ag, was shown to correlate with the IFN responsiveness of the five lines measured in antiproliferative and antiviral assays. Three of the lines, SK-MEL-28 (IFN sensitive), SK-MEL-3 (moderately IFN sensitive), and MM96 (IFN insensitive) were analyzed further to ..