Surface markers of a purified peritoneal eosinophil population from Mesocestoides corti-infected BALB/c male mice

Abstract

Eosinophils of approximately 95% purity were prepared from the peritoneal cavities of BALB/c male mice infected with the larval cestode, Mesocestoides corti. The alloantigenic surface marker phenotype of this cell population was shown to be H-2+LY4+Ly5+Lyt-1-,2-,3-Ly-6-,7-Ia-Thy-1-Tl-. Two of four anti-Lyt-2 sera were positive when tested on purified eosinophils by using the Staphylococcus aureus protein A sheep erythrocyte rosetting method, but absorption studies indicated that this reaction was not due to anti-Lyt-2 antibodies. Eosinophils are therefore Lyt-2-, although some Lyt-2 sera contain additional eosinophil reactive antibodies. A proportion (20 to 40%) of the population of eosinoph..