EVIDENCE THAT THY-1 AND LY-5 (T-200) ANTIGENS INTERACT WITH SULFATED CARBOHYDRATES

Abstract

Recent studies have demonstrated that lymphocytes express an array of cell surface receptors for sulphated polysaccharides (SP). Experiments were undertaken to determine the binding characteristics of these receptors and establish whether any known lymphocyte cell surface antigens interact with sulphated carbohydrates. It was found that murine thymocytes lack receptors for chondroitin-4-sulphate but express saturable, high affinity binding sites for heparin, fucoidan and dextran sulphate, with an apparent affinity constant range of 0.03-2.6 x 10(-9) mol/l. Binding inhibition experiments revealed one class of binding sites on murine thymocytes that is shared by heparin, fucoidan and dextran s..