Journal article
RECOMBINANT SOLUBLE RECEPTORS FOR THE FC-GAMMA PORTION INHIBIT ANTIBODY-PRODUCTION INVITRO
N VARIN, C SAUTES, A GALINHA, J EVEN, PM HOGARTH, WH FRIDMAN
EUROPEAN JOURNAL OF IMMUNOLOGY | WILEY | Published : 1989
Abstract
The problem of the structural relationship between suppressive IgG-binding factor and low-affinity receptors for the Fc portion of IgG (Fc gamma RII) has not yet been solved. In the present work we have isolated a recombinant soluble Fc gamma RII containing only the two external domains of Fc gamma RII, and analyzed its biochemical characteristics and biological activity. A cDNA encoding Fc gamma RII was mutated by the creation of a stop codon at the Lys175 codon. L cells have been transfected with this cDNA inserted into an expression vector. A cell line was obtained that secretes recombinant soluble Fc gamma RII which reacts with a monoclonal anti-Fc gamma RII antibody and binds to IgG1, I..
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