Journal article
Characterization of bovine heparin-binding neurotrophic factor (HBNF): Assignment of disulfide bonds
L Fabri, EC Nice, LD Ward, H Maruta, AW Burgess, RJ Simpson
Biochemistry International | ACADEMIC PRESS AUST | Published : 1992
Abstract
The topology of the disulfides in native heparin-binding neurotrophic factor (HBNF) isolated from bovine brain was studied by proteolytic digestion using trypsin, Asp-N endoproteinase and chymotrypsin and peptide mapping. Disulfide-linked peptides were identified by automated Edman degradation. It has been shown that there are disulfide bonds between Cys15-Cys44, Cys23-Cys53, Cys30-Cys57, Cys67-Cys99 and Cys77-Cys109.