Journal article

Human Granulocyte-Macrophage Colony-Stimulating Factor (hGM-CSF): Identification of a Binding Site for a Neutralizing Antibody

Edouard Nice, Peter Dempsey, Judith Layton, George Morstyn, Da-Fu Cui, Richard Simpson, Louis Fabri, Antony Burgess

Growth Factors | TAYLOR & FRANCIS LTD | Published : 1990

Abstract

One approach to the localization of functionally active regions of human granulocyte-macrophage colony-stimulating factor (hGM-CSF) is to map the epitopes recognized by neutralizing anti-hGM-CSF monoclonal antibodies. We have defined the epitope recognized by one neutralizing antibody (LMM102) using proteolytic fragments obtained by enzymic digestion of bacterially synthesized hGM-CSF. RP-HPLC fractionation of a tryptic digest resulted in the identification of an immunoreactive "tryptic core" peptide containing 66 amino acids (52% of the protein). Further digestion of this "tryptic core" with S. aureus V8 protease produced a unique immunoreactive hGM-CSF product comprising two peptides, resi..

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