Journal article

CD271 Expression on Patient Melanoma Cells Is Unstable and Unlinked to Tumorigenicity

Samantha E Boyle, Clare G Fedele, Vincent Corbin, Elisha Wybacz, Pacman Szeto, Jeremy Lewin, Richard J Young, Annie Wong, Robert Fuller, John Spillane, David Speakman, Simon Donahoe, Miklos Pohl, David Gyorki, Michael A Henderson, Ricky W Johnstone, Anthony T Papenfuss, Mark Shackleton

CANCER RESEARCH | AMER ASSOC CANCER RESEARCH | Published : 2016

Abstract

The stability of markers that identify cancer cells that propagate disease is important to the outcomes of targeted therapy strategies. In human melanoma, conflicting data exist as to whether hierarchical expression of CD271/p75/NGFR (nerve growth factor receptor) marks cells with enriched tumorigenicity, which would compel their specific targeting in therapy. To test whether these discrepancies relate to differences among groups in assay approaches, we undertook side-by-side testing of published methods of patient-derived melanoma xenografting (PDX), including comparisons of tissue digestion procedures or coinjected Matrigel formulations. We found that CD271(-) and CD271(+) melanoma cells f..

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