Journal article

Mammary Epithelial Reconstitution with Gene-Modified Stem Cells Assigns Roles to Stat5 in Luminal Alveolar Cell Fate Decisions, Differentiation, Involution, and Mammary Tumor Formation

Vida Vafaizadeh, Petra Klemmt, Christian Brendel, Kristoffer Weber, Carmen Doebele, Kara Britt, Manuel Grez, Boris Fehse, Sylvane Desrivieres, Bernd Groner

STEM CELLS | WILEY | Published : 2010

Abstract

The mammary gland represents a unique model system to study gene functions in adult stem cells. Mammary stem cells (MaSCs) can regenerate a functional epithelium on transplantation into cleared fat pads. We studied the consequences of distinct genetic modifications of MaSCs on their repopulation and differentiation ability. The reconstitution of ductal trees was used as a stem cell selection procedure and the nearly quantitative lentiviral infection efficiency of the primary mammary epithelial cells (MECs) rendered the enrichment of MaSCs before their transplantation unnecessary. The repopulation frequency of transduced MaSCs was nearly 100% in immunodeficient recipients and the resulting tr..

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Grants

Awarded by Deutsche Forschungsgemeinschaft (DFG)


Awarded by Medical Research Council


Funding Acknowledgements

This work was supported by grants from the Deutsche Forschungsgemeinschaft (DFG, GR 536/9-2 and GR 536/11-1). We thank the CIEA (Kawasaki, Japan) for the Rag2<SUP>-/-</SUP>gamma<INF>c</INF><SUP>-/-</SUP> mice. We are grateful to Dr. R. Moriggl (Ludwig Boltzmann Institute for Cancer Research, Vienna) for providing cS5-F, Dr. M. van der Valk (The Netherlands Cancer Institute, Amsterdam) for advice regarding pathology, and Dr. G. Robinson (National Institutes of Health, Bethesda) for fat pad clearing instructions.