Journal article

Plasmid DNA breakage by decal of DNA-associated Auger emitters: experiments with I-123/I-125-iodoHoechst 33258

PN Lobachevsky, RF Martin

International Journal of Radiation Biology | TAYLOR & FRANCIS LTD | Published : 2004

Abstract

PURPOSE: The Auger emitting isotope 123I has a much shorter half-life (13.2 hours), than 125I, the prototype Auger emitter. Monte Carlo simulations and cell culture studies indicate that decay of 123I covalently incorporated into DNA is about half as effective as 125I in terms of DNA breakage and cytotoxicity. The aim of the present study is to assess the DNA breakage efficacy of 123I that is non-covalently associated with DNA, using the minor groove binding ligand iodoHoechst 33258. MATERIALS AND METHODS: Plasmid (pBR322) DNA was incubated with mixtures of [123I]- and [125I]-iodoHoechst 33258, and DNA double strand breakage (DSB) assessed by assaying the relative amounts of intact, relaxed ..

View full abstract

University of Melbourne Researchers