gamma-radiation-induced gamma H2AX formation occurs preferentially in actively transcribing euchromatic loci
Raja S Vasireddy, Tom C Karagiannis, Assam El-Osta
CELLULAR AND MOLECULAR LIFE SCIENCES | SPRINGER BASEL AG | Published : 2010
The central dogma in radiation biology is that nuclear DNA is the critical target with respect to radiosensitivity. In accordance with the theoretical expectations, and in the absence of a conclusive model, the general consensus in the field has been to view chromatin as a homogeneous template for DNA damage and repair. This paradigm has been called into question by recent findings indicating a disparity in gamma-irradiation-induced gammaH2AX foci formation in euchromatin and heterochromatin. Here, we have extended those studies and provide evidence that gammaH2AX foci form preferentially in actively transcribing euchromatin following gamma-irradiation.
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Awarded by National Health and Medical Research Council of Australia
The support of the Australian Institute of Nuclear Science and Engineering is acknowledged. T.C.K. was the recipient of AINSE awards and is supported by the National Health and Medical Research Council of Australia (566559). R. V. is supported by a Melbourne Research Scholarship from the University of Melbourne.