Clonogenic assay: adherent cells.
Haloom Rafehi, Christian Orlowski, George T Georgiadis, Katherine Ververis, Assam El-Osta, Tom C Karagiannis
J Vis Exp | Published : 2011
The clonogenic (or colony forming) assay has been established for more than 50 years; the original paper describing the technique was published in 1956. Apart from documenting the method, the initial landmark study generated the first radiation-dose response curve for X-ray irradiated mammalian (HeLa) cells in culture. Basically, the clonogenic assay enables an assessment of the differences in reproductive viability (capacity of cells to produce progeny; i.e. a single cell to form a colony of 50 or more cells) between control untreated cells and cells that have undergone various treatments such as exposure to ionising radiation, various chemical compounds (e.g. cytotoxic agents) or in other ..View full abstract