UDP glucuronosyltransferase in the cirrhotic rat liver.
HS Debinski, PI Mackenzie, CS Lee, ML Mashford, JA Danks, TR Tephly, M Green, PV Desmond
J Gastroenterol Hepatol | Published : 1996
In patients with cirrhosis, the elimination of drugs metabolized by glucuronidation is relatively preserved, in comparison with the metabolism of drugs by oxidation. This study explores this phenomenon at a molecular level. In cirrhotic rat livers the content of UDP-glucuronosyltransferase (UGT) was examined by immunohistochemistry and immunoblotting using three antibodies: (i) a polyclonal antibody directed against a broad number of UGT isoforms from both family 1 and family 2; (ii) a family 2-specific antibody; and a (iii) family 1-specific antibody. The steady state mRNA level of UGT of a family 2 isoform was also detected by northern blot analysis. The results demonstrate normal or incre..View full abstract