Journal article
Regulation of amino acid transporters in pluripotent cell populations in the embryo and in culture; novel roles for sodium-coupled neutral amino acid transporters
BSN Tan, PD Rathjen, AJ Harvey, DK Gardner, J Rathjen
Mechanisms of Development | ELSEVIER SCIENCE BV | Published : 2016
Abstract
The developmental outcomes of preimplantation mammalian embryos are regulated directly by the surrounding microenvironment, and inappropriate concentrations of amino acids, or the loss of amino acid-sensing mechanisms, can be detrimental and impact further development. A specific role for L-proline in the differentiation of embryonic stem (ES) cells, a cell population derived from the blastocyst, has been shown in culture. L-proline acts as a signalling molecule, exerting its effects through cell uptake and subsequent metabolism. Uptake in ES cells occurs predominantly through the sodium-coupled neutral amino acid transporter 2, Slc38a2 (SNAT2). Dynamic expression of amino acid transporters ..
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Funding Acknowledgements
The authors would like to thank Dr. Marnie Blewitt and Wendy Allen for access to the WA30 ES cell line, Professor Paul Robson (Jackson Laboratories) for the trophectoderm stem cell line and Thi Thanhmai Troung for assistance with embryo collection. This work was supported by the University of Melbourne. AJH is supported by Stem Cells Australia, and BSNT was supported by an Endeavour International Postgraduate Research Scholarship and a Melbourne International Research Scholarship.