Journal article

Dense small molecule labeling enables activator-dependent STORM by proximity mapping

Ye Chen, Min Gu, Peter W Gunning, Sarah M Russell

HISTOCHEMISTRY AND CELL BIOLOGY | SPRINGER | Published : 2016

Abstract

Stochastic optical reconstruction microscopy (STORM) enables high-resolution imaging, but multi-channel 3D imaging is problematic because of chromatic aberrations and alignment errors. The use of activator-dependent STORM in which spectrally distinct activators can be coupled with a single reporter can circumvent such issues. However, the standard approach of linking activators and reporters to a single antibody molecule is hampered by low labeling density and the large size of the antibody. We proposed that small molecule labels might enable activator-dependent STORM if the reporter or activator were linked to separate small molecules that bound within 3.5 nm of each other. This would great..

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Grants

Awarded by Australian National Health and Medical Research Foundation


Funding Acknowledgements

We thank Sara Jones (Harvard University) for technical advice on STORM and Andrew Clayton (Swinburne University of Technology) for helpful discussions. The work was supported by a project grant from the Australian National Health and Medical Research Foundation (APP1061647), the Kids Cancer Project to PWG, and fellowships to SMR from the Australian Research Council (Future Fellowship) and the Australian National Health and Medical Research Foundation (Senior Research Fellowship).