Journal article

Assessing the performance of multiplexed tandem PCR for the diagnosis of pathogenic genotypes of Theileria orientalis using pooled blood samples from cattle

Hagos Gebrekidan, Robin B Gasser, Mark A Stevenson, Sean McGrath, Abdul Jabbar

MOLECULAR AND CELLULAR PROBES | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | Published : 2017

Abstract

Oriental theileriosis caused by multiple genotypes of Theileria orientalis is an important tick-borne disease of bovines. Here, we assessed the performance of an established multiplexed tandem PCR (MT-PCR) for the diagnosis of the two recognized, pathogenic genotypes (chitose and ikeda) of T. orientalis in cattle using pooled blood samples. We used a total of 265 cattle blood samples, which were divided into two groups according to previous MT-PCR results for individual samples. Samples in group 1 (n = 155) were from a herd with a relatively high prevalence of T. orientalis infection; and those in group 2 (n = 110) were from four herds with a low prevalence. For group 1, 31 and 15 batches of..

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Grants

Awarded by Water Quality Research Australia


Awarded by Melbourne Water Corporation


Funding Acknowledgements

This project was partially supported by a Collaborative Research Grant (the University of Melbourne) (A.J.) and the Australian Research Council (ARC) (R.B.G. et al.). H.G. is a grateful recipient of the Melbourne International Research Scholarships (MIRS) and Melbourne International Fee Remission Scholarships (MIFRS) through The University of Melbourne. The Easy-Plex platform (AusDiagnostics) used in this project was purchased from project 1043 (Dr Aaron R. Jex), funded by Water Quality Research Australia and Melbourne Water Corporation. We thank farmers for donating bovine blood samples for this study.