Recognition of the Major Histocompatibility Complex (MHC) Class Ib Molecule H2-Q10 by the Natural Killer Cell Receptor Ly49C
Lucy C Sullivan, Richard Berry, Natasha Sosnin, Jacqueline ML Widjaja, Felix A Deuss, Gautham R Balaji, Nicole L LaGruta, Michiko Mirams, Joseph A Trapani, Jamie Rossjohn, Andrew G Brooks, Daniel M Andrews
Journal of Biological Chemistry | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | Published : 2016
Murine natural killer (NK) cells are regulated by the interaction of Ly49 receptors with major histocompatibility complex class I molecules (MHC-I). Although the ligands for inhibitory Ly49 were considered to be restricted to classical MHC (MHC-Ia), we have shown that the non-classical MHC molecule (MHC-Ib) H2-M3 was a ligand for the inhibitory Ly49A. Here we establish that another MHC-Ib, H2-Q10, is a bona fide ligand for the inhibitory Ly49C receptor. H2-Q10 bound to Ly49C with a marginally lower affinity (∼5 μm) than that observed between Ly49C and MHC-Ia (H-2K(b)/H-2D(d), both ∼1 μm), and this recognition could be prevented by cis interactions with H-2K in situ To understand the molecula..View full abstract
This work was supported by National Health and Medical Research Council (NHMRC) Career Development Awards (to L. C. S., R. B, and D. M. A.), the Sylvia and Charles Viertel Charitable Foundation (to N. L L), and by an NHMRC Australia Fellowship (to J. R.). The authors declare that they have no conflicts of interest with the contents of this article.