Epitope Mapping of Avian Influenza M2e Protein: Different Species Recognise Various Epitopes
Noor Haliza Hasan, Esmaeil Ebrahimie, Jagoda Ignjatovic, Simson Tarigan, Anne Peaston, Farhid Hemmatzadeh
PLoS One | PUBLIC LIBRARY SCIENCE | Published : 2016
A common approach for developing diagnostic tests for influenza virus detection is the use of mouse or rabbit monoclonal and/or polyclonal antibodies against a target antigen of the virus. However, comparative mapping of the target antigen using antibodies from different animal sources has not been evaluated before. This is important because identification of antigenic determinants of the target antigen in different species plays a central role to ensure the efficiency of a diagnostic test, such as competitive ELISA or immunohistochemistry-based tests. Interest in the matrix 2 ectodomain (M2e) protein of avian influenza virus (AIV) as a candidate for a universal vaccine and also as a marker ..View full abstract
Awarded by Australian Centre for International Agricultural Research ACIAR
This research is funded by Australian Centre for International Agricultural Research ACIAR (http://aciar.gov.au/) grant numbers AH/2006/050 and AH/2010/039. NHH was funded by the Ministry of Education Malaysia. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.