Journal article

19-NOR DEOXYCORTICOSTERONE (19-NOR DOC) - MINERALOCORTICOID RECEPTOR AFFINITY HIGHER THAN ALDOSTERONE, ELECTROLYTE ACTIVITY LOWER

JW FUNDER, J MERCER, B INGRAM, D FELDMAN, K WYNNE, WR ADAM

ENDOCRINOLOGY | ENDOCRINE SOC | Published : 1978

Abstract

By screening urine extracts from rats with adrenal regeneration hypertension, Gomez-Sanchez et al. found a steroid, subsequently identified as 19-nor DOC, with high affinity for tritiated aldosterone (3HA) binding sites in rat kidney cytosol. We here report studies on the affinity of authentic 19-nor DOC for mineralocorticoid receptors, its binding in plasma and its activity in the rat urinary mineralocorticoid assay. When kidney slices from adrenalectomized rats were incubated in protein-free buffer with 3HA, 19-nor DOC consistently competed better (approximately 140%) for 3HA binding sites than did equivalent concentrations of non-radioactive aldosterone. Under identical conditions, save f..

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