Endocytosis and intracellular processing of platelet microparticles by brain endothelial cells
Dorothee Faille, Fatima El-Assaad, Andrew J Mitchell, Marie-Christine Alessi, Giovanna Chimini, Thierry Fusai, Georges E Grau, Valery Combes
Journal of Cellular and Molecular Medicine | WILEY | Published : 2012
Platelet-derived microparticles (PMP) bind and modify the phenotype of many cell types including endothelial cells. Recently, we showed that PMP were internalized by human brain endothelial cells (HBEC). Here we intend to better characterize the internalization mechanisms of PMP and their intracellular fate. Confocal microscopy analysis of PKH67-labelled PMP distribution in HBEC showed PMP in early endosome antigen 1 positive endosomes and in LysoTracker-labelled lysosomes, confirming a role for endocytosis in PMP internalization. No fusion of calcein-loaded PMP with HBEC membranes was observed. Quantification of PMP endocytosis using flow cytometry revealed that it was partially inhibited b..View full abstract
Awarded by National Health and Medical Research Council of Australia (NHMRC)
Awarded by Australian Research Council (ARC)
Awarded by European Union-Health and Medical Research Council of Australia (EU-NHMRC)
The authors are indebted to the Plate-forme d'Imagerie Commune Scientifique de Luminy for expert technical assistance. This work was supported by grants from the National Health and Medical Research Council of Australia (NHMRC Project Grant No. 464893; www.nhmrc.gov.au), the Australian Research Council (ARC Discovery Project No. DP0774425; www.arc.gov.au) and from the European Union-Health and Medical Research Council of Australia (EU-NHMRC Project Grant No. 512101; europa.eu), the Rebecca L. Cooper Foundation (www.cooperfoundation.org.au), the University of Sydney Major Equipment Grant scheme (www.usyd.edu.au) and the AL Kerr Bequest, Sydney Medical School. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.