Journal article

Endothelial Cells Potentiate Interferon-gamma Production in a Novel Tripartite Culture Model of Human Cerebral Malaria

Loke Tim Khaw, Helen J Ball, Jacob Golenser, Valery Combes, Georges E Grau, Julie Wheway, Andrew J Mitchell, Nicholas H Hunt

PLoS One | PUBLIC LIBRARY SCIENCE | Published : 2013

Abstract

We have established a novel in vitro co-culture system of human brain endothelial cells (HBEC), Plasmodium falciparum parasitised red blood cells (iRBC) and peripheral blood mononuclear cells (PBMC), in order to simulate the chief pathophysiological lesion in cerebral malaria (CM). This approach has revealed a previously unsuspected pro-inflammatory role of the endothelial cell through potentiating the production of interferon (IFN)-γ by PBMC and concurrent reduction of interleukin (IL)-10. The IFN-γ increased the expression of CXCL10 and intercellular adhesion molecule (ICAM)-1, both of which have been shown to be crucial in the pathogenesis of CM. There was a shift in the ratio of IL-10:IF..

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University of Melbourne Researchers