Ascorbate-deficient vtc2 mutants in Arabidopsis do not exhibit decreased growth
B Lim, N Smirnoff, CS Cobbett, JF GOLZ
Frontiers in Plant Science | Frontiers Media | Published : 2016
In higher plants the L-galactose pathway represents the major route for ascorbate biosynthesis. The first committed step of this pathway is catalyzed by the enzyme GDP-L-galactose phosphorylase and is encoded by two paralogs in Arabidopsis – VITAMIN C2 (VTC2) and VTC5. The first mutant of this enzyme, vtc2-1, isolated via an EMS mutagenesis screen, has approximately 20–30% of wildtype ascorbate levels and has been reported to have decreased growth under standard laboratory conditions. Here, we show that a T-DNA insertion into the VTC2 causes a similar reduction in ascorbate levels, but does not greatly affect plant growth. Subsequent segregation analysis revealed the growth defects of vtc2-1..View full abstract
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Awarded by Biotechnology and Biological Sciences Research Council
Awarded by BBSRC
This research is supported by a grant from the Australian Research Council (BL, CSC, JFG) and a Biotechnology and Biological Sciences Research Council grant (NS; BB/G021678/1).