Journal article

Understanding Factors That Modulate the Establishment of HIV Latency in Resting CD4 T-Cells In Vitro

Jenny L Anderson, Talia M Mota, Vanessa A Evans, Nitasha Kumar, Simin D Rezaei, Karey Cheong, Ajantha Solomon, Fiona Wightman, Paul U Cameron, Sharon R Lewin

PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2016

Abstract

Developing robust in vitro models of HIV latency is needed to better understand how latency is established, maintained and reversed. In this study, we examined the effects of donor variability, HIV titre and co-receptor usage on establishing HIV latency in vitro using two models of HIV latency. Using the CCL19 model of HIV latency, we found that in up to 50% of donors, CCL19 enhanced latent infection of resting CD4+ T-cells by CXCR4-tropic HIV in the presence of low dose IL-2. Increasing the infectious titre of CXCR4-tropic HIV increased both productive and latent infection of resting CD4+ T-cells. In a different model where myeloid dendritic cells (mDC) were co-cultured with resting CD4+ T-..

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Grants

Awarded by National Health and Medical Research Council of Australia Program Grant


Awarded by Division of AIDS, National Institute of Allergy and Infectious Diseases, US National Institutes of Health (Delaney AIDS Research Enterprise, DARE)


Funding Acknowledgements

This study was funded by a National Health and Medical Research Council of Australia Program Grant 1052979 (http://www.nhmrc.gov.au). SRL, PUC and JLA are supported by the Division of AIDS, National Institute of Allergy and Infectious Diseases, US National Institutes of Health (Delaney AIDS Research Enterprise, DARE; U19AI096109, http://www.niaid.nih.gov). SRL is also a National Health and Medical Research Council of Australia Practitioner Fellow (http://www.nhmrc.gov.au). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.