Journal article

CHARACTERIZATION OF A SALMONELLA-TYPHIMURIUM-ARO VACCINE STRAIN EXPRESSING THE P.69 ANTIGEN OF BORDETELLA-PERTUSSIS

R STRUGNELL, G DOUGAN, S CHATFIELD, I CHARLES, N FAIRWEATHER, J TITE, JL LI, J BEESLEY, M ROBERTS

Infection and Immunity | AMER SOC MICROBIOLOGY | Published : 1992

Abstract

The P.69 Bordetella pertussis protective antigen was expressed by use of the trc promoter from the chromosome of a Salmonella typhimurium aro vaccine strain, BRD509, by integrating the prn gene, encoding the 93-kDa precursor of this protein, into the aroC locus. P.69 was detected on the cell surface of the S. typhimurium strain (BRD640) by agglutination and immunoelectron microscopy. BALB/c mice immunized orally or intravenously with BRD640 showed a significant level of protection against an aerosol challenge with virulent B. pertussis, compared with control animals. No anti-P.69 antibodies in the serum or anti-P.69 antibody-secreting cells in the lungs were detected in BRD640-vaccinated ani..

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