Journal article

Predicted Coverage and Immuno-Safety of a Recombinant C-Repeat Region Based Streptococcus pyogenes Vaccine Candidate

Celia McNeilly, Samantha Cosh, Therese Vu, Jemma Nichols, Anna Henningham, Andreas Hofmann, Anne Fane, Pierre R Smeesters, Catherine M Rush, Louise M Hafner, Natkuman Ketheesan, Kadaba S Sriprakash, David J McMillan

PLoS One | PUBLIC LIBRARY SCIENCE | Published : 2016

Abstract

The C-terminal region of the M-protein of Streptococcus pyogenes is a major target for vaccine development. The major feature is the C-repeat region, consisting of 35-42 amino acid repeat units that display high but not perfect identity. SV1 is a S. pyogenes vaccine candidate that incorporates five 14mer amino acid sequences (called J14i variants) from differing C-repeat units in a single recombinant construct. Here we show that the J14i variants chosen for inclusion in SV1 are the most common variants in a dataset of 176 unique M-proteins. Murine antibodies raised against SV1 were shown to bind to each of the J14i variants present in SV1, as well as variants not present in the vaccine. Anti..

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University of Melbourne Researchers

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Funding Acknowledgements

This work was funded by the National Health and Medical Research Council of Australia and National Heart Foundation. The funders had no role in the study design, data analysis, and preparation of the manuscript or decision to submit the article for publication.This work was funded by the National Health and Medical Research Council of Australia and National Heart Foundation. The funders had no role in the study design, data analysis, and preparation of the manuscript or decision to submit the article for publication. Members of the M-protein study group provided the isolates that emm-sequences were derived from for analysis of the distribution of J14<INF>i</INF>.