Innate phagocytosis by peripheral blood monocytes is altered in Alzheimer’s disease

BJ Gu; X Huang; A Ou; A Rembach; C Fowler; PK Avula; A Horton; JD Doecke; VL Villemagne; SL Macaulay; P Maruff; EL Fletcher; R Guymer; JS Wiley; CL Masters; The Australian Imaging, Biomarkers

Journal article

Innate phagocytosis by peripheral blood monocytes is altered in Alzheimer’s disease

BJ Gu, X Huang, A Ou, A Rembach, C Fowler, PK Avula, A Horton, JD Doecke, VL Villemagne, SL Macaulay, P Maruff, EL Fletcher, R Guymer, JS Wiley, CL Masters, undefined The Australian Imaging, Biomarkers

Acta Neuropathologica | SPRINGER | Published : 2016

DOI: 10.1007/s00401-016-1596-3

Abstract

Sporadic Alzheimer’s disease (AD) is characterised by the deposition and accumulation of specific protein aggregates. Failure of clearance could underlie this process, and recent genetic association studies point towards involvement of the phagocytosis and autophagy pathways. We developed a real-time tri-color flow cytometry method to quantitate the phagocytic function of human peripheral blood monocyte subsets including non-classic CD14dimCD16+, intermediate CD14+CD16+ and classic CD14+CD16− monocytes. Using this method, we have measured the phagocytic ability of fresh monocytes in a study of preclinical, prodromal and clinical AD, matched with cognitively normal healthy control subjects. B..

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University of Melbourne Researchers

Victor Villemagne Author

Paul Maruff Author

Erica Fletcher Author

Robyn Guymer Author

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Grants

Awarded by Australian Research Council

Funding Acknowledgements

We thank Dr. Verena Wimmer for the help of confocal microscopy. A full list of AIBL investigators can be found at http://aibl.csiro.au/about/aibl-research-team. This work was supported by ARC Future Fellowship (BG, FT120100581) and NHMRC Project Grant (1048082, 1061419), Macular Disease Foundation Australia Project Grant (2014-2016), Macular Society Grant USA (2015) and the Victorian Government's Operational Infrastructure Support Grant to the Florey Institute.