Journal article

MONOCLONAL-ANTIBODIES SPECIFIC FOR THE CORE PROTEIN OF THE BETA-SUBUNIT OF THE GASTRIC PROTON PUMP (H /K ATPASE) - AN AUTOANTIGEN TARGETED IN PERNICIOUS-ANEMIA

CM JONES, BH TOH, JM PETTITT, TM MARTINELLI, DC HUMPHRIS, JM CALLAGHAN, I GOLDKORN, FT MU, PA GLEESON

The FEBS Journal | SPRINGER VERLAG | Published : 1991

Abstract

The gastric H+/K(+)-transporting adenosine triphosphatase (H+/K+ ATPase) (proton pump) consists of a catalytic alpha-subunit and a recently proposed 60-90-kDa glycoprotein beta-subunit. Using dog gastric membranes as the antigen, we have produced two murine monoclonal antibodies, 4F11 (IgG1) and 3A6 (IgA), which are specific for the 60-90-kDa glycoprotein. The monoclonal antibodies (1) specifically stained the cytoplasm of unfixed and formalin-fixed dog gastric parietal cells; (2) specifically reacted by ELISA with gastric tubulovesicular membranes; (3) recognised epitopes located on the luminal face of parietal cell tubulovesicular membranes, the site of the proton pump, by immunogold elect..

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