Culture of Dermal Papilla Cells from Ovine Wool Follicles: An In Vitro Model for Papilla Size Determination
Nicholas W Rufaut, Allan J Nixon, Rodney D Sinclair, RM Hoffman (ed.)
MULTIPOTENT STEM CELLS OF THE HAIR FOLLICLE: METHODS AND PROTOCOLS | SPRINGER | Published : 2016
Common human balding or hair loss is driven by follicle miniaturization. Miniaturization is thought to be caused by a reduction in dermal papilla size. The molecular mechanisms that regulate papilla size are poorly understood, and their elucidation would benefit from a tractable experimental model. We have found that dermal papilla cells from sheep spontaneously aggregate in culture to form papilla-like structures. Here, we describe methods for microdissecting dermal papillae from wool follicles, for initiating and maintaining cultures of ovine papilla cells, and for using these cells in an in vitro assay to measure the effect of bioactive molecules on aggregate size.