Journal article
Development and evaluation of a multi-antigen peptide ELISA for the diagnosis of Chlamydia trachomatis-related infertility in women
Shruti Menon, Scott H Stansfield, Benignus Logan, Jane S Hocking, Peter Timms, Luk Rombauts, John A Allan, Wilhelmina M Huston
JOURNAL OF MEDICAL MICROBIOLOGY | MICROBIOLOGY SOC | Published : 2016
DOI: 10.1099/jmm.0.000311
Abstract
Chlamydia trachomatis results in tubal factor infertility in some women. Diagnosis of this tubal infertility is difficult and typically involves laparoscopy or hysterosalpingography to detect the tubal blockages. Numerous serological tests have been developed; however, they are presently not used for diagnosis without subsequent surgical investigation during the infertility investigation. This study aimed to develop a highly specific serological assay for chlamydial tubal factor infertility in women that could be used to recommend direct progression to invitro fertilization (IVF) treatment for women who are positive. Women were recruited from a variety of settings including women seeking fer..
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Funding Acknowledgements
The authors want to thank and acknowledge all of the participants who kindly gave their time to contribute to the research study. The study nurses and research officers were extremely valuable for recruitment and data collection, especially Caroline Motteram, Deborah de Guingand and Helen Woodhouse. The authors wish to thank the research nurses, clinic nurses and staff that facilitated our study, consented participants and collected data (Monash IVF Clayton and Monash IVF Wesley). The authors also thank the staff and participants at the Sexual Health Clinics especially Dr John Patten, Dr John Dwyer, Dr Joseph Debattista and all the clinical staff of the Brisbane Sexual Health Clinic; Dr Stuart Aitken, Dr Maree O'Sullivan, Brenda Henry and all the clinical staff of the Gold Coast Sexual Health; Dianne Farrell and all staff of the S.H.op 101; Dr Kuong Taing, Karen McGill and all the staff of the Clinic 87 Nambour Sexual Health; Dr Darren Russell and Collette Cashman and all the staff of the Cairns Sexual Health Clinic; and the QUT University Health Service Medical Centre staff especially Leonie O'Keefe and the staff and medical students at the various antenatal clinics and practices that facilitated recruitment of pregnant participants. The collection and storage of some serological specimens were conducted in conjunction with the Wesley Medical Research Institute Tissue Bank. The study has been funded by the Wesley Medical Medical Research Institute grants awarded to J.A.A. and W.M.H., the Queensland Government National and International Research Alliance Project awarded to P. T. (sub-project leader W. H.), the Monash IVF Group Research and Education Foundation funding awarded to L. R. and W. H. and the QUT Bluebox Proof of Concept Funding. Several of the authors are named inventors on a patent that has been filed in Australia for this assay. The patent is owned by the QUT BLUEBOX and not by any of the authors.