Journal article
Changes in the Cytoplasmic Composition of Amino Acids and Proteins Observed in Staphylococcus aureus during Growth under Variable Growth Conditions Representative of the Human Wound Site
Mousa M Alreshidi, R Hugh Dunstan, Johan Gottfries, Margaret M Macdonald, Marcus J Crompton, Ching-Seng Ang, Nicholas A Williamson, Tim K Roberts
PLoS One | Public Library of Science (PLoS) | Published : 2016
Abstract
Staphylococcus aureus is an opportunistic pathogen responsible for a high proportion of nosocomial infections. This study was conducted to assess the bacterial responses in the cytoplasmic composition of amino acids and ribosomal proteins under various environmental conditions designed to mimic those on the human skin or within a wound site: pH6-8, temperature 35–37°C, and additional 0–5% NaCl. It was found that each set of environmental conditions elicited substantial adjustments in cytoplasmic levels of glutamic acid, aspartic acid, proline, alanine and glycine (P< 0.05). These alterations generated characteristic amino acid profiles assessed by principle component analysis (PCA). Substant..
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Awarded by University of Newcastle, Australia funds
Funding Acknowledgements
This work was supported by the Ministry of Education and University of Hail, Saudi Arabia. MA This was a PhD Scholarship. This was also supported by Harold Stannet Williams and Judith Mason Research Foundation (http://www.anz.com.au/resources/2/f/2fce2b804a47773887c8cfac93b0266b/MasonNatMedGuidelines.pdf?CACHEID=2fce2b804a47773887c8cfac93b0266b), Gideon Lang Research Foundation and the University of Newcastle, (grant no. G0189306) Australia funds for post-graduate research. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.r Mousa Alreshidi was supported by the Ministry of education and University of Ha'il, Saudi Arabia. This work was also supported by the University of Newcastle, (grant no. G0189306) Australia funds for post-graduate research as well as the Harold Stannet Williams and Judith Mason Research Foundation and the Gideon Lang Research Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. http://www.anz.com.au/resources/2/f/2fce2b804a47773887c8cfac93b0266b/MasonNatMedGuidelines.pdf?CACHEID=2fce2b804a47773887c8cfac93b0266b