Posttranslational modifications and dysfunction of mitochondrial enzymes in human heart failure
Freya L Sheeran, Salvatore Pepe
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM | AMER PHYSIOLOGICAL SOC | Published : 2016
Deficiency of energy supply is a major complication contributing to the syndrome of heart failure (HF). Because the concurrent activity profile of mitochondrial bioenergetic enzymes has not been studied collectively in human HF, our aim was to examine the mitochondrial enzyme defects in left ventricular myocardium obtained from explanted end-stage failing hearts. Compared with nonfailing donor hearts, activity rates of complexes I and IV and the Krebs cycle enzymes isocitrate dehydrogenase, malate dehydrogenase, and aconitase were lower in HF, as determined spectrophotometrically. However, activity rates of complexes II and III and citrate synthase did not differ significantly between the tw..View full abstract
Awarded by National Health and Medical Research Council of Australia (NHMRC)
F. Sheeran was supported by the National Health and Medical Research Council of Australia (NHMRC Dora Lush Postgraduate Scholar) and an Early Career PostDoctoral Fellowship (GNT1016543). This work was supported in part by NHMRC Project funding (S. Pepe) and the Victorian Government's Operational Infrastructure Support Program to the Murdoch Childrens Research Institute.