Cytogenetic analysis of eight human papillomavirus immortalized human keratinocyte cell lines

Abstract

Cytogenetic analysis was performed on human papillomavirus (HPV)‐immortalized cell lines. Lines were established by co‐transfection of primary human keratinocyte cells with HPV type 16 or 18 DNA and pSV2neo. The resulting clonal lines contained integrated HPV DNA and exhibited extended life spans in culture but were non‐tumorigenic in nude mice. Two HPV16‐immortaiized lines (FEPEIL8 and FEPEIL9) and 4 HPV18‐immortalized lines (FEA, FEH18L, FEP18‐5 and FEP18‐11) were established. Two additional lines were derived by subsequent treatment of the FEA line with TPA and by further transfection with HSVII DNA. Cytogenetic analysis revealed that all lines were abnormal, containing a variety of numer..