Transfer of mRNA encoding recombinant immunoreceptors reprograms CD4( ) and CD8( ) T cells for use in the adoptive immunotherapy of cancer
K Birkholz, A Hombach, C Krug, S Reuter, M Kershaw, E Kaempgen, G Schuler, H Abken, N Schaft, J Doerrie
Gene Therapy | NATURE PUBLISHING GROUP | Published : 2009
Awarded by Friedrich-Alexander-University Erlangen-Nuremberg
Awarded by DFG-German Research Foundation
We thank Tanja de Gruijl and Saskia Santegoets (VU University Medical Center, Amsterdam) for providing us with the KATO III cell line, Kris Thielemans (Medical School of the Vrije Universiteit Brussel, Brussels) for providing the pGEM4Z-5'UTR-sig-MAGE-A3-DC. LAMP-3'UTR RNA production vector. We thank Tanja Schunder, Verena Wellner, and Stefanie Baumann for excellent technical assistance. We thank Stefanie Hoyer and Christian Hofmann for fruitful discussions. Work in the Erlangen laboratory was financially supported by the ELAN-Funds of the Friedrich-Alexander-University Erlangen-Nuremberg (DE-07.09.12.1) and the DFG-German Research Foundation (SCHA 1247/1-1). Work in the Cologne laboratory was supported by the European Community through ATTACK and by the Fortune program of the Medical Faculty.