Design and Use of Peptide Substrates for Protein Kinases

Abstract

This chapter focuses on the design and use of peptide substrates for protein kinases. There are three approaches to the design of protein kinase peptide substrates. The most widely used approach is to synthesize analogs of known phosphorylation site sequences. These may be from natural substrates, autophosphorylation sites, or phosphorylation sites in exogenous substrates such as histone, myelin basic protein, or caseins. The second approach is to use random polymers of amino acids such as Tyr and Glu for tyrosine kinases. The third approach is to prepare substrate analogs of the pseudosubstrate autoregulatory regions that have been found in a number of protein kinases. Synthetic peptide sub..