Journal article
Structural dissection of a complex Bacteroides ovatus gene locus conferring xyloglucan metabolism in the human gut
GR Hemsworth, AJ Thompson, J Stepper, ŁF Sobala, T Coyle, J Larsbrink, O Spadiut, ED Goddard-Borger, KA Stubbs, H Brumer, GJ Davies
Open Biology | ROYAL SOC | Published : 2016
DOI: 10.1098/rsob.160142
Abstract
The human gastrointestinal tract harbours myriad bacterial species, collectively termed the microbiota, that strongly influence human health. Symbiotic members of our microbiota play a pivotal role in the digestion of complex carbohydrates that are otherwise recalcitrant to assimilation. Indeed, the intrinsic human polysaccharide-degrading enzyme repertoire is limited to various starch-based substrates; more complex polysaccharides demand microbial degradation. Select Bacteroidetes are responsible for the degradation of the ubiquitous vegetable xyloglucans (XyGs), through the concerted action of cohorts of enzymes and glycan-binding proteins encoded by specific xyloglucan utilization loci (X..
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Awarded by Australian Research Council
Funding Acknowledgements
Work in the Davies group was supported by the BBSRC (grant no. BB/I014802/1), L.S. is supported by the European Research Council proposal No. 322942-'GlycoPOISE'. Work in the Brumer group during the course of this project was supported by the Mizutani Foundation for Glycoscience, The Swedish Research Council Formas (via CarboMat-the KTH Advanced Carbohydrate Materials Centre), The Swedish Research Council (Vetenskapsradet), the Knut and Alice Wallenberg Foundation (via the Wallenberg Wood Science Centre), faculty funding from the University of British Columbia, the Natural Sciences and Engineering Research Council of Canada (Discovery Grant), the Canada Foundation for Innovation and the British Columbia Knowledge Development Fund, and the Canadian Institutes for Health Research (MOP-137134, MOP-142472). Support for this work by the Australian Research Council (K.A.S.), the Australian Government, the University of Western Australia, and the Centre for Microscopy, Characterisation and Analysis at the University of Western Australia (T.C.) is also acknowledged.