Journal article
Haplotyping the human leukocyte antigen system from single chromosomes
Nicholas M Murphy, Matthew Burton, David R Powell, Fernando J Rossello, Don Cooper, Abha Chopra, Ming Je Hsieh, David C Sayer, Lavinia Gordon, Mark D Pertile, Brian D Tait, Helen R Irving, Colin W Pouton
SCIENTIFIC REPORTS | NATURE PUBLISHING GROUP | Published : 2016
DOI: 10.1038/srep30381
Abstract
We describe a method for determining the parental HLA haplotypes of a single individual without recourse to conventional segregation genetics. Blood samples were cultured to identify and sort chromosome 6 by bivariate flow cytometry. Single chromosome 6 amplification products were confirmed with a single nucleotide polymorphism (SNP) array and verified by deep sequencing to enable assignment of both alleles at the HLA loci, defining the two haplotypes. This study exemplifies a rapid and efficient method of haplotyping that can be applied to any chromosome pair, or indeed all chromosome pairs, using a single sorting operation. The method represents a cost-effective approach to complete phasin..
View full abstract