Journal article

Elevated paternal glucocorticoid exposure alters the small noncoding RNA profile in sperm and modifies anxiety and depressive phenotypes in the offspring

AK Short, KA Fennell, VM Perreau, A Fox, MK O'Bryan, JH Kim, TW Bredy, TY Pang, AJ Hannan



Recent studies have suggested that physiological and behavioral traits may be transgenerationally inherited through the paternal lineage, possibly via non-genomic signals derived from the sperm. To investigate how paternal stress might influence offspring behavioral phenotypes, a model of hypothalamic-pituitary-adrenal (HPA) axis dysregulation was used. Male breeders were administered water supplemented with corticosterone (CORT) for 4 weeks before mating with untreated female mice. Female, but not male, F1 offspring of CORT-treated fathers displayed altered fear extinction at 2 weeks of age. Only male F1 offspring exhibited altered patterns of ultrasonic vocalization at postnatal day 3 and,..

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Awarded by NHMRC project

Awarded by ARC FT3 Future Fellowship

Funding Acknowledgements

We thank Ms Shanshan Li and Dr Xin Du for assistance in tissue collection, Ms Stephanie Smith for technical assistance with male fertility assays and Christopher Butler for proofreading. RNA-sequencing work was supported through the Illumina Pilot Grant Program. This work was funded through an NHMRC project grant to AJH, TWB and TYP (APP1083468), an ARC FT3 Future Fellowship to AJH (FT100100835) and supported by the Victorian Government through the Operational Infrastructure Scheme. AJH is an NHMRC Senior Research Fellow.