Journal article

Divergent Transcriptional Responses to Physiological and Xenobiotic Stress in Giardia duodenalis

Brendan RE Ansell, Malcolm J McConville, Louise Baker, Pasi K Korhonen, Samantha J Emery, Staffan G Svard, Robin B Gasser, Aaron R Jex

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | AMER SOC MICROBIOLOGY | Published : 2016

Abstract

Understanding how parasites respond to stress can help to identify essential biological processes. Giardia duodenalis is a parasitic protist that infects the human gastrointestinal tract and causes 200 to 300 million cases of diarrhea annually. Metronidazole, a major antigiardial drug, is thought to cause oxidative damage within the infective trophozoite form. However, treatment efficacy is suboptimal, due partly to metronidazole-resistant infections. To elucidate conserved and stress-specific responses, we calibrated sublethal metronidazole, hydrogen peroxide, and thermal stresses to exert approximately equal pressure on trophozoite growth and compared transcriptional responses after 24 h o..

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