Role of the CD39/CD73 Purinergic Pathway in Modulating Arterial Thrombosis in Mice
Roman Covarrubias, Elena Chepurko, Adam Reynolds, Zachary M Huttinger, Ryan Huttinger, Katherine Stanfill, Debra G Wheeler, Tatiana Novitskaya, Simon C Robson, Karen M Dwyer, Peter J Cowan, Richard J Gumina
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY | LIPPINCOTT WILLIAMS & WILKINS | Published : 2016
OBJECTIVE: Circulating blood cells and endothelial cells express ectonucleoside triphosphate diphosphohydrolase-1 (CD39) and ecto-5'-nucleotidase (CD73). CD39 hydrolyzes extracellular ATP or ADP to AMP. CD73 hydrolyzes AMP to adenosine. The goal of this study was to examine the interplay between CD39 and CD73 cascade in arterial thrombosis. APPROACH AND RESULTS: To determine how CD73 activity influences in vivo thrombosis, the time to ferric chloride-induced arterial thrombosis was measured in CD73-null mice. In response to 5% FeCl3, but not to 10% FeCl3, there was a significant decrease in the time to thrombosis in CD73-null mice compared with wild-type mice. In mice overexpressing CD39, ab..View full abstract
Awarded by National Heart, Lung, and Blood Institute of the National Institutes of Health
Awarded by NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
Awarded by NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
This work was supported in part by the National Heart, Lung, and Blood Institute of the National Institutes of Health through training grant 5T32HL007411-35 to R. Covarrubias, R01-HL094400 to S.C. Robson and grants K08-HL094703 and R21-HL096038 to R.J. Gumina.