Journal article
Comprehensive Analysis of the SUL1 Promoter of Saccharomyces cerevisiae
MS Rich, C Payen, AF Rubin, GT Ong, MR Sanchez, N Yachie, MJ Dunham, S Fields
Genetics | Oxford University Press | Published : 2016
Abstract
In the yeast Saccharomyces cerevisiae, beneficial mutations selected during sulfate-limited growth are typically amplifications of the SUL1 gene, which encodes the high-affinity sulfate transporter, resulting in fitness increases of >35%. Cis-regulatory mutations have not been observed at this locus; however, it is not clear whether this absence is due to a low mutation rate such that these mutations do not arise, or they arise but have limited fitness effects relative to those of amplification. To address this question directly, we assayed the fitness effects of nearly all possible point mutations in a 493-base segment of the gene’s promoter through mutagenesis and selection. While most mut..
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Awarded by National Institutes of Health
Funding Acknowledgements
We thank Bill Noble and Charles Grant for help with Tomtom and FIMO and for discussions about motif matching and Kunihiro Ohta for the use of his tetrad dissection microscope. This work was supported by grants R01 GM094306 (to M.J.D.) and P41 GM103533 (to M.J.D. and S.F.) from the National Institute of General Medical Sciences of the National Institutes of Health. M.J.D. is a Rita Allen Foundation Scholar and a Senior Fellow in the Genetic Networks program at the Canadian Institute for Advanced Research. S.F. is an investigator of the Howard Hughes Medical Institute. M.S.R. was partially supported by a Short-term Postdoctoral Fellowship from the Japan Society for the Promotion of Science.