Journal article

Using SANS with Contrast-Matched Lipid Bicontinuous Cubic Phases To Determine the Location of Encapsulated Peptides, Proteins, and Other Biomolecules

Leonie van 't Hag, Liliana de Campo, Christopher J Garvey, George C Feast, Anna E Leung, Nageshwar Rao Yepuri, Robert Knott, Tamar L Greaves, Tran Nhiem, Sally L Gras, Calum J Drummond, Charlotte E Conn



An understanding of the location of peptides, proteins, and other biomolecules within the bicontinuous cubic phase is crucial for understanding and evolving biological and biomedical applications of these hybrid biomolecule-lipid materials, including during in meso crystallization and drug delivery. While theoretical modeling has indicated that proteins and additive lipids might phase separate locally and adopt a preferred location in the cubic phase, this has never been experimentally confirmed. We have demonstrated that perfectly contrast-matched cubic phases in D2O can be studied using small-angle neutron scattering by mixing fully deuterated and hydrogenated lipid at an appropriate ratio..

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University of Melbourne Researchers


Awarded by ARC Dairy Innovation Hub

Awarded by ARC DECRA Fellowship

Funding Acknowledgements

We thank Prof. Raffaele Mezzenga for fruitful discussions on the project. We thank Yesim Gozukara for help with DSC measurements. We acknowledge support of the Bragg Institute, Australian Nuclear Science and Technology Organisation in providing neutron research facilities and the Australian National Deuteration Facility (partly supported by the National Collaborative Research Infrastructure Strategy (NCRIS) - an initiative of the Australian Government) for providing the chemical deuteration facilities used in this work. We acknowledge use of the SAXS/WAXS beamline at the Australian Synchrotron and we thank Drs. Adrian M. Hawley, Nigel M. Kirby, and Stephen T. Mudie for their assistance. We thank AINSE, Ltd for providing financial assistance (Award - PGRA) to L.v.H. to enable work at the Bragg Institute. S.L.G. is supported by the ARC Dairy Innovation Hub IH 120100005. C.E.C. is the recipient of an ARC DECRA Fellowship (DE160101281).