Journal article
HER3 and downstream pathways are involved in colonization of brain metastases from breast cancer
L Da Silva, PT Simpson, CE Smart, S Cocciardi, N Waddell, A Lane, BJ Morrison, AC Vargas, S Healey, J Beesley, P Pakkiri, S Parry, N Kurniawan, L Reid, P Keith, P Faria, E Pereira, A Skalova, M Bilous, RL Balleine Show all
Breast Cancer Research | BMC | Published : 2010
DOI: 10.1186/bcr2603
Open access
Abstract
Introduction: Metastases to the brain from breast cancer have a high mortality, and basal-like breast cancers have a propensity for brain metastases. However, the mechanisms that allow cells to colonize the brain are unclear.Methods: We used morphology, immunohistochemistry, gene expression and somatic mutation profiling to analyze 39 matched pairs of primary breast cancers and brain metastases, 22 unmatched brain metastases of breast cancer, 11 non-breast brain metastases and 6 autopsy cases of patients with breast cancer metastases to multiple sites, including the brain.Results: Most brain metastases were triple negative and basal-like. The brain metastases over-expressed one or more membe..
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Funding Acknowledgements
Leonard Da Silva and Ana Cristina Vargas are recipients of PhD Fellowships from the Ludwig Institute of Cancer Research. Leonard Da Silva is enrolled with the "Universidade Federal de Sao Paulo, Escola Paulista de Medicina, Curso de Pos-Graduacao, Doutorado, Departamento de Anatomia Patologica, Sao Paulo, Brazil". Peter Simpson is a recipient of a fellowship from the National Breast Cancer Foundation. Georgia Chenevix-Trench and KumKum Khanna are Senior Principal Research Fellows of the NHMRC. RLB is a Cancer Institute NSW Fellow. We also acknowledge the help of staff within anatomical pathology, RBWH, Brisbane, the animal house facility at UQ AIBN, Brisbane, Casey Wright from the Thoracic Research Laboratory, School of Medicine, at the UQ, and Clay Winterford and his staff from the UQ/QIMR Histotechnology facility, and Macky Edmundson in the sequencing facility at QIMR. We would like to thank Sequenom Inc. for providing the primer sequences used for HRM, and, in particular, we thank Darryl Irwin for his help.