Journal article

Proteomics of muscle chronological ageing in post-menopausal women

Marine Gueugneau, Cecile Coudy-Gandilhon, Ophelie Gourbeyre, Christophe Chambon, Lydie Combaret, Cecile Polge, Daniel Taillandier, Didier Attaix, Bertrand Friguet, Andrea B Maier, Gillian Butler-Browne, Daniel Bechet



BACKGROUND: Muscle ageing contributes to both loss of functional autonomy and increased morbidity. Muscle atrophy accelerates after 50 years of age, but the mechanisms involved are complex and likely result from the alteration of a variety of interrelated functions. In order to better understand the molecular mechanisms underlying muscle chronological ageing in human, we have undertaken a top-down differential proteomic approach to identify novel biomarkers after the fifth decade of age. RESULTS: Muscle samples were compared between adult (56 years) and old (78 years) post-menopausal women. In addition to total muscle extracts, low-ionic strength extracts were investigated to remove high abu..

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Awarded by European Union Collaborative Project MyoAge

Awarded by Fondation Caisse d'Epargne Rhone-Alpes (CERA Sarcopenie)

Awarded by Fonds Europeens de Developpement Regional (FEDER)

Awarded by FEDER

Funding Acknowledgements

This work was partly funded by the European Union Collaborative Project MyoAge (EC Fp7 CT-223576) and by the Fondation Caisse d'Epargne Rhone-Alpes (CERA Sarcopenie no 30). MG was supported by a postgraduate fellowship from Region Auvergne and Fonds Europeens de Developpement Regional (FEDER, no 23000422), and LT by a postdoctoral fellowship from Region Auvergne and FEDER (no 35380 T2a 2011 Prenusa). The authors wish to gratefully acknowledge Professor Philippe Courpron for his constant support and his central role in initiating and developing this work. The authors thank the Fondation pour l'Universite de Lyon for assistance with obtaining reagents.