Journal article

Targeted Biomarker Discovery by High Throughput Glycosylation Profiling of Human Plasma Alpha1-Antitrypsin and Immunoglobulin A

L Renee Ruhaak, Carolien AM Koeleman, Hae-Won Uh, Jord C Stam, Diana van Heemst, Andrea B Maier, Jeanine J Houwing-Duistermaat, Paul J Hensbergen, P Eline Slagboom, Andre M Deelder, Manfred Wuhrer

PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2013

Abstract

Protein N-glycosylation patterns are known to show vast genetic as well as physiological and pathological variation and represent a large pool of potential biomarkers. Large-scale studies are needed for the identification and validation of biomarkers, and the analytical techniques required have recently been developed. Such methods have up to now mainly been applied to complex mixtures of glycoproteins in biofluids (e.g. plasma). Here, we analyzed N-glycosylation profiles of alpha1-antitrypsin (AAT) and immunoglobulin A (IgA) enriched fractions by 96-well microtitration plate based high-throughput immuno-affinity capturing and N-glycan analysis using multiplexed capillary gel electrophoresis..

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Grants

Awarded by IOP (innovation oriented programs, The Netherlands)


Awarded by European Union (Seventh Framework Programme HighGlycan project,


Funding Acknowledgements

This work was funded by IOP (innovation oriented programs, The Netherlands) grant IGE05007, and by the European Union (Seventh Framework Programme HighGlycan project, grant number: 278535). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.