Journal article

ANALYSIS OF ANDROGEN ACTION ON PITUITARY GONADOTROPIN AND PROLACTIN SECRETION IN EWES

IJ CLARKE, K MITCHELHILL, E ZACHARIAH, JK FINDLAY, JW FUNDER

BIOLOGY OF REPRODUCTION | SOC STUDY REPRODUCTION | Published : 1982

Abstract

To study the role of androgens in the control of gonadotropin and prolactin secretion in ther ewe, we have characterized androgen receptors in pituitary cytosol, and investigated the effect of androgens on pituitary hormone release in vivo and in vitro. High affinity, low capacity receptors, with an affinity for methyltrienolone (R1881) greater than 5 alpha-dihydrotestosterone (5 alpha-DHT) greater than testosterone (T) much greater than androstenedione (A4), estradiol-17 beta (E2) and progesterone (P), were identified in pituitary cytosol. Addition of 1 nM 5 alpha-DHT, but not A4, inhibited luteinizing hormone (LH) release from pituitary cells in vitro, induced by 10(10) to 10(-7) M luteini..

View full abstract

University of Melbourne Researchers