Screening of the 'Pathogen Box' identifies an approved pesticide with major anthelmintic activity against the barber's pole worm
Sarah Preston, Yaqing Jiao, Abdul Jabbar, Sean L McGee, Benoit Laleu, Paul Willis, Timothy NC Wells, Robin B Gasser
INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE | ELSEVIER SCI LTD | Published : 2016
There is a substantial need to develop new medicines against parasitic diseases via public-private partnerships. Based on high throughput phenotypic screens of largely protozoal pathogens and bacteria, the Medicines for Malaria Venture (MMV) has recently assembled an open-access 'Pathogen Box' containing 400 well-curated chemical compounds. In the present study, we tested these compounds for activity against parasitic stages of the nematode Haemonchus contortus (barber's pole worm). In an optimised, whole-organism screening assay, using exsheathed third-stage (xL3) and fourth-stage (L4) larvae, we measured the inhibition of larval motility, growth and development of H. contortus. We also stu..View full abstract
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Awarded by Victoria Life Sciences Computation Initiative, Australia (VLSCI)
Awarded by University of Melbourne
The present study was funded by the National Health and Medical Research Council of Australia (NHMRC), the Australian Research Council (ARC) and the Wellcome Trust (RBG), and supported by a Victoria Life Sciences Computation Initiative, Australia (VLSCI; grant no. VR0007) on its Peak Computing Facility at The University of Melbourne, Australia, an initiative of the Victorian Government, Australia. Animal ethics approval (AEC no. 0707258) was granted by The University of Melbourne. We thank our colleagues at MMV, especially Angelique Doy, for their support.