Journal article
Anti-HIV antibody responses and the HIV reservoir size during antiretroviral therapy
SA Lee, P Bacchetti, N Chomont, R Fromentin, SR Lewin, U O'Doherty, S Palmer, DD Richman, JD Siliciano, SA Yukl, SG Deeks, PD Burbelo
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2016
Abstract
Background: A major challenge to HIV eradication strategies is the lack of an accurate measurement of the total burden of replication-competent HIV (the "reservoir"). We assessed the association of anti-HIV antibody responses and the estimated size of the reservoir during antiretroviral therapy (ART). Methods: We evaluated anti-HIV antibody profiles using luciferase immunoprecipitation systems (LIPS) assay in relation to several blood-based HIV reservoir measures: total and 2-LTR DNA (rtPCR or droplet digital PCR); integrated DNA (Alu PCR); unspliced RNA (rtPCR), multiply-spliced RNA (TILDA), residual plasma HIV RNA (single copy PCR), and replication-competent virus (outgrowth assay). We als..
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Awarded by National Institute of Diabetes and Digestive and Kidney Diseases
Funding Acknowledgements
This work was supported in part by the National Institutes of Health (1K23GM112526, 1R01DK108349, the DARE Collaboratory [U19 AI096109], the CARE Collaboratory [U19 AI096113]), the Division of Intramural Research of the National Institutes, the National Institute of Dental and Craniofacial Research, and the National Health and Medical Research Council of Australia. This work was also supported by the amfAR Research Consortium on HIV Eradication a.k.a. ARCHE (108072-50-RGRL), the Department of Veterans Affairs (IK2 CX000520), the UCSD Center for AIDS Research (P30 AI36214), and the James B. Pendleton Charitable Trust. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.