Journal article

The N-terminal pro-domain of the kalata B1 cyclotide precursor is intrinsically unstructured

Norelle L Daly, Sunithi Gunasekera, Richard J Clark, Feng Lin, John D Wade, Marilyn A Anderson, David J Craik

BIOPOLYMERS | WILEY | Published : 2016

Abstract

Cyclotides are plant-derived, gene-encoded, circular peptides with a range of host-defense functions, including insecticidal activity. They also have potential as pharmaceutical scaffolds and understanding their biosynthesis is important to facilitate their large-scale production. Insights into the biosynthesis of cyclotides are emerging but there are still open questions, particularly regarding the influence of the structure of the precursor proteins on processing/biosynthetic pathways. The precursor protein of kalata B1, encoded by the plant Oldenlandia affinis, contains N- and C-terminal propeptides that flank the mature cyclotide domain. The C-terminal region (ctr) is important for the c..

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Grants

Awarded by Australian Research Council (ARC) Future Fellowships


Funding Acknowledgements

NLD and RJC acknowledge the support of Australian Research Council (ARC) Future Fellowships (FT110100222 and FT100100476, respectively). DJC is an ARC Australian Laureate Fellow (FL150100146). During these studies, JDW was an NHMRC Principal Research Fellow (5018148). Work at the Florey Institute of Neuroscience and Mental Health was supported by the Victorian Government's Operational Infrastructure Support Program