Journal article

Chemical Derivatization and Ultrahigh Resolution and Accurate Mass Spectrometry Strategies for "Shotgun" Lipidome Analysis

Eileen Ryan, Gavin E Reid

Accounts of Chemical Research | AMER CHEMICAL SOC | Published : 2016


Lipids play critical structural and functional roles in the regulation of cellular homeostasis, and it is increasingly recognized that the disruption of lipid metabolism or signaling or both is associated with the onset and progression of certain metabolically linked diseases. As a result, the field of lipidomics has emerged to comprehensively identify and structurally characterize the diverse range of lipid species within a sample of interest and to quantitatively monitor their abundances under different physiological or pathological conditions. Mass spectrometry (MS) has become a critical enabling platform technology for lipidomic researchers. However, the presence of isobaric (i.e., same ..

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University of Melbourne Researchers


Awarded by National Institutes of Health

Awarded by Australian Research Council

Funding Acknowledgements

Funding was provided from the National Institutes of Health (Grant GM103508) and the Australian Research Council (Grant DP130100535). We thank former members of the Reid lab, Dr. Cassie Fhaner, Dr. Todd Lydic, and Dr. Shuai Nie, who were largely responsible for initial development of the derivatization chemistries and lipidomics workflow described herein.