Chemical Derivatization and Ultrahigh Resolution and Accurate Mass Spectrometry Strategies for "Shotgun" Lipidome Analysis
Eileen Ryan, Gavin E Reid
Accounts of Chemical Research | AMER CHEMICAL SOC | Published : 2016
Lipids play critical structural and functional roles in the regulation of cellular homeostasis, and it is increasingly recognized that the disruption of lipid metabolism or signaling or both is associated with the onset and progression of certain metabolically linked diseases. As a result, the field of lipidomics has emerged to comprehensively identify and structurally characterize the diverse range of lipid species within a sample of interest and to quantitatively monitor their abundances under different physiological or pathological conditions. Mass spectrometry (MS) has become a critical enabling platform technology for lipidomic researchers. However, the presence of isobaric (i.e., same ..View full abstract
Awarded by National Institutes of Health
Awarded by Australian Research Council
Funding was provided from the National Institutes of Health (Grant GM103508) and the Australian Research Council (Grant DP130100535). We thank former members of the Reid lab, Dr. Cassie Fhaner, Dr. Todd Lydic, and Dr. Shuai Nie, who were largely responsible for initial development of the derivatization chemistries and lipidomics workflow described herein.